- 結(jié)構(gòu)基因
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structured gene中文什么意思
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- Expression of human papillomavirus type 16 l1 structure gene in sf9 cells and its products assembling into virus - like particles
1在昆蟲細(xì)胞中的表達(dá)及其生物學(xué)活性 - Now , the structure gene sequence and 3 ' - end gene sequence have been logged on genbank . the accession number is ay044918
目前，此過敏蛋白tb22kda的結(jié)構(gòu)基因與3 ’端基因序列已在genbank登錄，登錄號為ay044918 。 - The promoter and structure genes of x phage lysis genes were amplified by pcr , respectively . the plasmid mvps was constructed for integrating the vhb gene and the lysis genes into the chromosome of e . colihms174 by ligating the lysis genes to mini - tn5
利用pcr擴(kuò)增得到噬菌體裂解基因的啟動子和結(jié)構(gòu)基因( srrz ) ，連接在轉(zhuǎn)座子minitn5上，構(gòu)建成為質(zhì)粒mvps ，用于將vhb基因和srrz基因共同插入到e . colihms174的染色體上。 - Technical features : adopt new technology fabrics seven results , with mature ceramic production technology , powder buwen various raw materials to be transparent , according to the natural stone structure genes , generating component fabrics , rich layers . natural stone texture stronger products
技術(shù)特點(diǎn):采用全新七級布料科技成果，結(jié)合成熟的陶瓷生產(chǎn)工藝技術(shù)，以各種粉料布紋加以透明原料，根據(jù)天然石材的構(gòu)造基因工程，生成通體布料、層次豐富、天然石材質(zhì)感更強(qiáng)產(chǎn)品 - According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well
本研究根據(jù)先前分離純化所得天然tb22kda蛋白經(jīng)maldi - tof - ms (質(zhì)譜法)測得的氨基酸序列和文獻(xiàn)報道的過敏蛋白核苷酸序列設(shè)計引物，擴(kuò)增克隆了該過敏蛋白結(jié)構(gòu)基因的編碼序列；根據(jù)測得的序列設(shè)計特異性引物，并利用3 ’ - race方法結(jié)合巢式pcr擴(kuò)增得到基因的3 ’末端；依據(jù)同源性比較的結(jié)果選用一段保守序列為5 ’引物，并根據(jù)結(jié)構(gòu)基因內(nèi)部序列設(shè)計3 ’特異性引物，進(jìn)一步獲得了該基因5 ’端的部分序列。